Improving chemo-enzymatic synthesis towards green and sustainable protein synthesis

Kiem GoChem

Chemo-enzymatic peptide synthesis is unique in enabling the fast and sustainable synthesis of cyclic peptides, complex peptides and functionalized mini-proteins.

The starting materials are routinely obtained by solid-phase peptide synthesis. One of the starting materials requires an oxo-ester functionality for recognition by the enzymes active site. The SPPS-based synthesis of the oxo-ester functionality still suffers from significant byproduct formation and low overall synthesis yields. In this project we are going to improve the synthesis of peptides with C-terminal oxo-esters.