K. Knoops
Already since his master in biology at the University of Groningen, Kèvin specialized in both light and electron microscopy. Afterwards, he completed his PhD thesis (“Nidovirus replication structures; Hijacking membranes to support viral RNA synthesis”) at the Leiden University Medical Center (LUMC) in which the replication organelles of Nidoviruses were studied using advanced cellular imaging techniques. Supported by an EMBO long-term fellowship, Kèvin moved to the EMBL in Grenoble in 2010 to study the structure of translating ribosomes with single-particle electron microscopy. In 2012, Kèvin moved back to Groningen and was awarded the Marie Curie Intra-European Fellowship as well as the NWO VENI to perform 5 years as post-doc to study the biogenesis and structure of yeast peroxisomes. In 2017, Kèvin finally moved to the Microscopy CORE lab at Maastricht University as microscopy specialist where he currently is responsible for the Light Microscopy section.
Microscopy skills
In vivo (confocal) fluorescence microscopy
Immune-fluorescence microscopy
Correlative light- and electron microscopy (CLEM)
AiryScan, spinning disc and multiphoton microscopy
Spectral imaging and linear unmixing
Stimulated emission depletion microscopy (STED)
High-throughput and –content image processing
Intensity- and object-based co-localization analysis
Chemical and cryo-fixation of cells, isolated organelles and protein complexes
Low-dose imaging
Freeze-substitution
Ultra-thin (serial) sectioning
Immunogold labeling
Dual-axis electron tomography, reconstruction and modelling
Electron spectroscopy imaging
Single-particle EM analysis
Sub-tomogram averaging
Molecular cell biology skills
Culturing of bacteria, yeast, mammalian cells and viruses
Genetic engineering of plasmid and genomic DNA
Protein and DNA characterization by standard biochemistry methods
Licensed to work with radionuclides (level 5B) and GMOs, viruses and bacteria at ML-2
Cell lysate fractionation and gradient floatation
Protein expression, isolation and purification
