Measuring Metabolic Engines and Fuels with the Agilent Seahorse XF Analyzer
Learn about Agilent’s new functional, kinetic, cell based assays targeting key disease areas and microbial analysis. Metabolism is the key to understanding cell function.
In living cells, most of the energy produced is derived from three fuel sources: glucose, glutamine, and fatty acids. Mitochondrial access to these fuels impacts a wide variety of biological processes. Use the Agilent Seahorse XF Analyzer to: o Identify fuel dependencies to uncover cancer cell vulnerabilities. o Explore how fuel preferences lead to cell fate decisions for differentiation and immune cell activation. o Determine whether/how cells can adjust fuel oxidation to match nutrient availability while meeting energy demand. o Distinguish metabolic adaptations due to genetic changes vs. those that take place due to nutrient deprivation.
Host Miranda Nabben, PhD m.nabben[at]maastrichtuniversity[dot]nl Maastricht University Medical Center
Presenter Dr. Daniel Gebhard daniel.gebhard[at]agilent[dot]com Product Specialist, Cell Analysis, Agilent
14:00 – 14:45 Introduction to Seahorse Technology Introduction and assay optimization
14:45 – 15:15 Metabolism in living cells with examples from different research focuses
15:15 – 15:35 Oxygen matters! Narrowing the in vitro - in vivo divide in cellular oxygenation
15:35 – 15:50 Size matters! Measuring metabolism in 3 D samples
15:50 – 16:00 Break
16:00 – 16:30 Characterizing modes of action with Isolated Mitochondria / Permeabilized Cells
16:30 – 17:15 Software and data analysis crash course on our data analysis software Seahorse Analytics
Agilent Cell Analysis Training and Seminar: PDF