Measuring Metabolic Engines and Fuels with the Agilent Seahorse XF Analyzer

In living cells, most of the energy produced is derived from three fuel sources: glucose, glutamine, and fatty acids. Mitochondrial access to these fuels impacts a wide variety of biological processes. Use the Agilent Seahorse XF Analyzer to: o Identify fuel dependencies to uncover cancer cell vulnerabilities. o Explore how fuel preferences lead to cell fate decisions for differentiation and immune cell activation. o Determine whether/how cells can adjust fuel oxidation to match nutrient availability while meeting energy demand. o Distinguish metabolic adaptations due to genetic changes vs. those that take place due to nutrient deprivation.

EVENT DETAILS
Host Miranda Nabben, PhD m.nabben@maastrichtuniversity.nl Maastricht University Medical Center
Presenter Dr. Daniel Gebhard daniel.gebhard@agilent.com Product Specialist, Cell Analysis, Agilent

AGENDA
14:00 – 14:45 Introduction to Seahorse Technology Introduction and assay optimization
14:45 – 15:15 Metabolism in living cells with examples from different research focuses
15:15 – 15:35 Oxygen matters! Narrowing the in vitro - in vivo divide in cellular oxygenation
15:35 – 15:50 Size matters! Measuring metabolism in 3 D samples
15:50 – 16:00 Break
16:00 – 16:30 Characterizing modes of action with Isolated Mitochondria / Permeabilized Cells
16:30 – 17:15 Software and data analysis crash course on our data analysis software Seahorse Analytics

Agilent Cell Analysis Training and Seminar: PDF