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Light microscopes

These are the currently available objectives of all microscopes.

Leica TCS SP8 STED

  • Fluorescence confocal laser scanning microscope with a stimulated emission depletion (STED) laser at 592nm for super-resolution fluorescence microscopy.
  • Contains a UV (405nm) and white-light laser (WLL2; tuneable from 470 to 670nm) to excite a wide variety of fluorescent proteins and probes.
  • For emission detection, it has two conventional PMTs as well as two hybrid HyD detectors with gating.

  • Fast image 3D image scanning can be achieved by combining the resonance scanner and Super Z Galvo stage, whereas the CO2 and temperature controlled incubator enables live-cell imaging.

     

  • Runs Leica LAS X software.
  • Acquired images can be processed with the SVI Huygens deconvolution software at a Windows work station.
Microscopy CORE Lab Leica STED microscope

Leica DM4000 B LED

  • Automated upright widefield fluorescence microscope with a manual stage and Leica EL6000 light source.
  • Equipped with a Perkin Elmer Nuance FX multispectral tissue imager that can be used for filter-free spectral unmixing of (auto-) fluorescence. 
Microscopy CORE Lab Leica DM LED microscope

FEI CorrSight

  • (Cryo-) fluorescence microscope specifically designed for correlative microscopy experiments
  • Can be used in both widefield and spinning-disc imaging mode.
  • Widefield imaging is achieved by a TILL Oligochrome light source, DAPI-FITC-TxRed Tripleband HC Filter and Hamamatsu 1k ORCA-R2, whereas the spinning-disc mode makes use of a Toptica iChrome MLE LFA laser module (405, 488, 561 and 640nm), an Andromeda Yokogawa spinning disk module and Hamamatsu 2k Flash 4V2plus camera.
  • The CorrSight cryostage enables observation of samples at cryogenic temperatures, whereas the Ibidi heated chamber plate and pump enable live-cell experiments.
  • Runs FEI MAPS software to overlay correlative fluorescence microscopy images directly onto SEM images.
Microscopy CORE Lab FEI Corrsight

Zeiss Axio Observer 7

  • Widefield inverted fluorescent microscope
  • Brightfield microscope
  • CO2, temperature and humidity controlled OKO Lab incubator for live-cell imaging
  • Combined CO2/Oxygen Controller for Hypoxia
  • Large tile scanning

  • Apotome 3 for optical sectioning in widefield fluorescent microscopy

     

  • Cameras:
    • Axiocam 705 monochrome
    • Axiocam 305 color

    • Perkin Elmer Nuance EX multispectral (450 nm – 950 nm) for spectral unmixing

       

  • Software
    • Zeiss ZEN
    • Nuance
Microscopy CORE Lab Zeiss Actio Observer

Evident FV4000 Multiphoton microscope + Refined lasers CARS/SRS

  • Multiphoton microscope
  • Refined CARS and SRS modules
  • Upright microscope
  • Gantry frame suitable from cell experiments to large experimental set-ups
  • Cryo stage
  • Fluorescent light source: LEDs at 367 nm, 407 nm, 436 nm, 470 nm and 550 nm
  • Okolab incubator for controlled temperature live-cell imaging
  • Camera: monochrome CMOS camera Qi2
  • Chameleon Discovery NX with TPC tunable laser from 660 nm to 1320 nm and fixed laser at 1040 nm
  • Forward and backward detectors, NDD detectors
  • Second and third harmonic generation
  • Software: cellSens