Esther Miguelez Barragan

  Faculty of Health, Medicine & Life Sciences | Bachelor Biomedical Sciences

Benchmarking instrumentation and analysis methods for relative quantification of complex proteomic samples

Esther's elevator pitch
Mass spectrometry is revolutionizing how we measure proteins in biological samples, thanks to cutting-edge tools like the Orbitrap Astral and advanced tagging technologies. This study compared the performance of the Astral with the currently used analyzers, and assessed a new correction algorithm for isobaric tagging errors. The Astral excelled at detecting low-abundance proteins and was more consistent across runs, but it struggled with precision, leading to higher error rates. Meanwhile, the correction algorithm improved accuracy but sacrificed precision. These advancements promise breakthroughs in biomarker discovery but underscore the need for fine-tuning instruments and methods to maximize both accuracy and precision in protein quantification.