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…  Which populations need to be identified in which tissue  What are the available instrument configurations/ fluorochromes BD FACS Canto BD Fusion sorter UV laser (355): 7 channels Violet laser (405): 18 channels Red laser (635): 6 channels Blue laser (488): 7 channels https://spectrum.cytekbio.com Cytek Aurora Most standard applications High autofluorescence Big panels (>8 markers) Highly similar fluorophores To sort out populations for further analysis: - RNA/protein extraction - Cell culture - … Research question Select antigens  Know your biological hypothesis  Select antigens: identify markers of interest  Expression level?  Co-expressed?  Gating strategy  Check literature! Marker Expression level Marker Co-expression Gating strategy FlowMetric.com Size/shape FSC vs SSC Peak uniformity Area vs Height Dead cell dye negative CD45+ CD3hi CD4+ CD8+ Think about how you want to “arrive” at your population … marker expression Janine Bögli Stimulation control Controls  FMO (Fluorescence Minus One)  Contains all the antibodies in the panel, minus one of them  Helpfull to define background, spread, autofluorescence and gate setting Clean up data  Spot spillover/compensation/unmixing errors -> weirdly shaped populations -> should be round -> extreme negatives -> a below 0 light signal does not exist -> biological impossibilities -> marker expression  Come to us for help Clean up data  Check …

… the information provided and make sure to complete all tasks as soon as possible (and definitely before the indicated deadlines). You can find an overview of important deadlines on the Admission requirements-page of this programme (see the dark-blue menu on the left-hand side of this page). 1. Phase 1: Studielink application Let us know you want to apply for this study programme Studielink To start the application process for this study programme, you first need to apply via Studielink. … a DigiD yet, you will need to request one (Apply for a DigiD). If you do not (yet) live in the Netherlands, you can register by creating a Studielink account (without DigiD). As your personal details will not have been verified through DigiD, UM will check your personal details. Apply via Studielink Please note You have to select your ‘starting moment’ in Studielink. Please make sure to select the right month and year in the dropdown-menu, so that you are applying for a start of the study programme … You will receive an email from UM within 24 hours of having applied in Studielink. This email contains your username/UM student number and a link to create your personal password. You can use these login details to access the MyApplication portal, the online environment in which the rest of your application process will take place. The https://www.maastrichtuniversity.nl/education/bachelor https://www.maastrichtuniversity.nl/education/bachelor/university-college-venlo https://www.digid.nl/en …

… distributed information in the file too, offering you a more complete overview of all information currently available. This document will also be uploaded to the O&O website. Maastricht University created general websites with information about online learning: Roadmaps and Tools for Teaching Staff and Online education at UM. Should you require help creating online lectures or tutorial meetings, you can contact the UM helpdesk via Helpdesk-onlinelearning@maastrichtuniversity.nl Index CHAPTER … organize breakout sessions; small breaks for working in subgroups (breakout rooms) or individually. The first tutorial session of a new block Welcome and test (5 -10 min): In the first tutorial you’ll have to plan time for a test session. You have to check whether all students have their cameras on, and microphones on muted. Also, discuss that students, if possible, use their cameras, since it is hard to interact with persons who are not visible. To have an overview of all students put your screen … how they are doing/ what activities they do at home etc. You may consider using breakout rooms to include exchanges in small groups. Divide roles: Divide roles as usual (e.g., who is chair, who is scribe). In Zoom you can make the chair your co-host (blue button, upper-right corner) Table: Ground rules Technical rules Process related rules Talk one by one. When you are not speaking put microphone on mute (left lower corner), this prevents background noise during the session. Be on time Indicate how …