Practicals Human Genetics, Reproduction
Practical 1: Anatomy - Blastocyst development/Implantation/Early development
Students will learn about the development during week 1-4 of embryonic development. They will study how to judge the adequate development during fetal and embryonic period.
Practical 2: Anatomy - Body Plan + Gut
Students will study general aspects of the body plan and will have a special look on the macroscopy of the gut system.
Practical 3: Anatomy - Sexual differentiation - virtual microscopy
Development of the gonads and differentiation of germ cells will be studied.
Practical 4: Anatomy sexual organs
Students will study the topography of the sexual organs in focusing on developmental aspects.
Practical 5: Devolpemt of the heart
Students will study the the heart in focusing on developmental aspects.
Lab practical 1: DNA isolation
Student will isolation of genomic DNA from buccal cells and prepare lab buffers for practical days 2 and 3.
Computer practical: Online Mendelian Inheritance in Man (OMIM) and NEBcutter
Student will learn how to browse and interpret OMIM entries and they will learn how to use NEBcutter for restriction enzyme analysis.
Lab practical 2: DNA concentration analysis and PCR
Student will analyze the concentration of their DNA and perform a Polymerase Reaction (PCR) for Clade Marker M270.
Lab practical 3: DNA visualization
Student will perform restriction enzyme digestion and will visualize the experimental products with gel electrophoresis
Doelstellingen van dit vak
- Understand the early human development and organogenesis
- Compare developing and adult structures.
- Be able to conclude what has been going wrong during development in some common anomalies.
- Be able to find online genetic information
- Perform some basic techniques to detect a monogenetic disease.
Embryology S.F. Gilbert: Developmental Biology (10th & 11th ed.). Sinauer Ass., Sunderland, 2006/2011. W. J. Larsen: Human Embryology, 3rd/4th edition (2001/2008) Moore and Persaud “The developing human” 8th edition 2008, 9th edition 2012 Langmans medical embryology 11th / 12th edition 2008/2011 Carlson “Human embryology and developmental biology” 5th edition Elsevier Saunders Films embryonic development (available via ELEUM anatomical site under “Course Materials”) Physiology Silverthorn DU: Human Physiology, 5th ed., 2009, Prentice Hall, Pearson, San Francisco, USA ; ISBN-13 9780321559395 / 6th edition 2012 / ISBN 9780321798619 Alberts et al.: Molecular Biology of the Cell. 5th ed. 2007) Garland Science Macroscopic anatomy - atlases Prometheus: Atlas of human anatomy Sobotta: Atlas of human anatomy Virtual microscopy A.L. Kierszenbaum: Histology and Cell Biology, an Introduction to Pathology. Mosby, St. Louis, 2002; 3e editie 2011 / ISBN 9780323078429 Ross, Pawlina: Histology a text and atlas (6th) Lippincott Williams and Wilkins Molecular biology Lewine 3rd edition: Cells Alberts, 6th edition, Molecular Biology of the Cell Lodish, 7th edition, Molecular Cell Biology: