Full course description
The skills trainings are aimed to obtain a basic introduction to techniques and methods in modern Genetics. The first skills take place at a designated skills laboratory at Chemelot campus; subsequent skills training topics “Genomes and Genomics” and are taught in a computer landscape. These days integrate theoretical and practical information. Each student will receive theoretical and practical in silico training in the morning, followed by a limited number of tasks to execute on the computer and answered in a skills report. The final skills consist of a student group presentation where the combined theoretical and practical skills on Genetics are applied to a pre-assigned task.
- To be able to purify genomic DNA from eukaryotic cells and plasmid (circular) DNA from prokaryotic cells and perform quantitative and qualitative analyses nucleic acid products.
- To isolate RNA from eukaryotic cells and apply reverse transcription to generate copy DNA.
- To perform and comprehend polymerase chain reaction (PCR) analysis.
- Basic cloning techniques: manipulation of DNA products using restriction digestion and ligation.
- To study specific proteins from tissue by Western immunoblotting.
- To present in-depth knowledge in an essay on predefined genetic concepts.
- BIO2001 Cell Biology
- BIO2007 Genetics
- “Introduction to Genetic Analysis” by Griffiths, Wessler, Carrol, Doebley (Palgrave Macmillan, 11thedition, 2015. ISBN-13: 9781464109485).
- The PowerPoint files from the BIO2007 lectures.