Advanced Microscopy: Theory and Applications
Full course description
Light microscopy is an established visualization method used in many fields as a standard analysis tool - from histopathological examination of biopsies, to observation of rare minerals’ optical properties. Technological advances in light sources and electronics have made possible the development of advanced imaging modalities. Such modalities can be used to image anything from the detailed structure of the nuclear pore, to the development of a whole zebrafish embryo in real time, with subcellular resolution. Advanced techniques include Confocal microscopy, Two-photon microscopy, Light Sheet microscopy and Super-resolution microscopy. In this course the advantages and disadvantages of each technique are going to be discussed and relevant applications are going to be presented. Practical sessions will help students get acquainted with such techniques and experience how microscopic images are created. Basic image processing of these images is going to be performed. This course is aimed at any student with an interest in imaging and its principles.
- To acquaint the student with an understanding of principles of optical microscopy and limiting factors in resolution.
- To introduce and detail a number of microscope techniques and the theory behind them.
- To discuss the factors that limit contrast, resolution, and penetration depth of these techniques.
- To explain sample preparation procedures.
- To have hands-on experience with each of the techniques.
- PHY1001 Elements of Physics or PHY1101 Introduction to Physics