Full course description
The general aim of this skills course is to obtain detailed knowledge about the techniques that can be applied to address molecular processes in mammalian biology. Topics include the activation of intracellular signaling pathways; analysis of cellular responses; analysis of gene expression; analysis of protein activation; in silico analysis of signaling pathways; and the culmination of the above elements in an essay and assignment to indicate active understanding of the above processes. The skills days are designed to provide a perspective of how molecular biological techniques are applied to tackle major research questions in modern biomedical research.
- To be able to investigate protein/nucleic acid interactions via an electrophoretic mobility shift assay (EMSA).
- To perform and comprehend a cytological staining and to visualize and interpret the results.
- To extract small nuclear RNA species, assess RNA quality and determine isolation efficiency.
- To isolate proteins from cells and perform protein quantification.
- To perform a chemotaxis assay and interpret the results.\
- To perform an immunoprecipitation (IP) and interpret the final results by Western immunoblotting.
- To put together a research grant proposal plan based on previously published research; to present and defend it towards peers and tutors.
- BIO2001 Cell Biology
- BIO2007 Genetics
- PRA2014 Genetics
- BIO3001 Molecular Biology
A reader is provided at the start of the course. Other recommended literature will be announced later.