This skill will contain: • Cell culture • Immunohistochemistry • qPCR • Metabolic assays
The main objective of this course is to provide a practical introduction into molecular and cell biology and to demonstrate how experimental cell biology can be used in the context of regenerative medicine. We will use osteoblasts to study the effects of a hypoxia mimic on angiogenic markers. A variety of experiments will be performed to study differences on RNA and protein level. Cell Profiler will be used for image/data analysis.
• Practical laboratory instructions. • A small molecule approach to engineering vascularized tissue. Doorn J1, Fernandes HA, Le BQ, van de Peppel J, van Leeuwen JP, De Vries MR, Aref Z, Quax PH, Myklebost O, Saris DB, van Blitterswijk CA, de Boer J. Biomaterials. 2013 Apr;34(12):3053-63