Practical Training: Immunocytochemical Staining of Human Postmortem Tissue and Evaluation of the Staining Using the Multihead Microscope
Full course description
An immunocytochemical procedure will be followed to label plaques (ABeta) and neurofibrillary tangles (abnormal Tau) and to the staining will be evaluated afterwards using the multihead microscope.
Students will be able to understand:
- collecting Postmortem tissue, fixation, paraffin, immunocytochemical staining, recognition of neuropathological hallmarks in Tauopathies: Alzheimer’s disease (AD);
- plaques, tangles Synucleinopathies: Parkinson disease, Multisystem atrophy;
- polyglutamine diseases: Huntington, and Spinocerebellar ataxias;
- mixed pathogies;
- diffuse Lewy body disease, early and late onset AD, Amyloid beta cascade hypothesis, amyloid precursor protein, Tau, ubiquitin, GFAP, gliosis, cytoarchitecture of hippocampus and neocortex.
28 Oct 2019
20 Dec 2019
Teaching methods:Lecture(s), PBL, Research, Skills, Training(s)
Assessment methods:Attendance, Observation, Take home exam
Keywords:Tauopathies (e.g. Alzheimer’s), synucleinopathies (e.g. Parkinson), polyglutamine diseases (Huntington), Neurodegenerative mechanisms