The general aim of this skills course is to obtain detailed knowledge about the techniques that can be applied to address molecular processes in cell signaling and control of gene expression. Topics include the activation of intracellular signaling pathways; analysis of cellular responses; analysis of gene expression; analysis of protein activation; in silico analysis of signaling pathways; and the culmination of the above elements in an essay and assignment to indicate active understanding of the above processes. The skills days are designed to provide a perspective of how cutting edge molecular biological techniques are applied to tackle major research questions in modern biomedical research.
• To grasp the contextual setting which animal models are commonly used in Molecular Life Sciences. • To apply DNA cloning, transfection and imaging procedures using prokaryotic and eukaryotic cells. • To perform quantitative analyses on (non)coding RNA species and proteins from cell culture and organ biopsies. • To apply molecular biological principles to a pre-assigned task and present the findings to a larger audience. • To interpret scientific results and to write a scientific proposal on a Molecular Biological approach to relevant human disorders and defend it in a larger audience.
A reader is provided at the start of the course. Recommended literature sources: "Molecular Cell Biology" by Lodish, Berk, Kaiser (W.H. Freeman, 7th edition, 2012. bol.com price €145) and "Introduction to Genetic Analysis" by Griffiths, Wessler, Carrol, Doebley (W.H. Freeman, 10th edition, International Edition, 2012. bol.com price €64,99).