The use of advanced imaging techniques in light and electron microscopy is wide-spread, particularly in the fields of material science and biological imaging. In recent years, the resolution and/or functionality of such tools has been increased due to the development of fluorescence microscopy, two-photon excitation microscopy, correlative light-electron microscopy and stimulation emission depletion (STED) microscopy [first experimentally shown by the winners of the 2014 Nobel Prize in Chemistry]. In electron microscopy, rapid developments in aberration correction and in image filtering allow users to understand much more about the samples they are investigating. This course will introduce a number of advanced imaging techniques to participants, detailing theoretical aspects as well as practical considerations. This course is aimed any students from the programme with an interest in imaging and its principles.
• To acquaint the student with an understanding of principles of (light) microscopy and limiting factors in resolution • To introduce and detail a number of microscope designs and theory as to how they overcome the resolution limit • Introduction and understanding of electron microscopy and its application to material science and biological imaging • To explain sample preparation procedures and perform a demonstration of some of the equipment
BIO1001 Introduction to Natural Sciences: Biology MAT1001 Introduction to Natural Sciences: Calculus or PHY1002 Introduction to Natural Sciences: Mathematical Foundations of Physics Recommended PHY1001 Elements of Physics